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Cooling breaks

 Due to the relatively high ambient temperatures in Brazil, particularly at the northern venues, cooling breaks for the players were introduced.[51] Breaks could take place at the referee's discretion after the 30th minute of each half if the Wet Bulb Globe Temperature exceeded 32 °C (90 °F); the breaks would last 3 minutes, with this time made up by an extended period of stoppage time at the end of the half.

The first cooling break in a World Cup play took place during the 32nd minute of the match between the Netherlands and Mexico in the round of 16.[52][53][54][55] At the start of the match, FIFA listed the temperature at 32 °C (90 °F) with 68% humidity.[56]

Anti-doping

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The biological passport was introduced in the FIFA World Cup starting in 2014. Blood and urine samples collected from all players before the competition, and from two players per team per match, were analysed by the Swiss Laboratory for Doping Analyses.[57] FIFA reported that 91.5% of the players taking part in the tournament were tested before the start of the competition and none tested positive.[58] However, FIFA was criticised for how it conducted doping tests.[59][60]

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